IISc

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Raghothama Group

NMR Research Center, IISc, Bangalore

 

Research Highlights

 

NMR analysis of natural and synthetically designed peptides:

NMR spectroscopy is a powerful technique for the study of conformational and structural aspects of biological molecules.  In the use of this methodology various approaches are possible. Of these, one of the important areas is the study of natural and synthetically designed peptides which have important roles in controlling biological processes. Currently scientific investigations are focused on the application of NMR spectroscopy to conformational and structural aspects of such biomolecules.  State of the art NMR methodologies have been applied for such studies and detailed structural analysis have been carried out.  The use of computer based molecular modeling and structure calculation have also been made.  Listed below are some of these studies carried out, part of which also form collaborative efforts:

            (i)                 Amyloid peptide fragment analysis.

            (ii)              Studies oriented towards drug design based on natural and synthetic peptides.

            (iii)            Applications of recent methodological developments in solid state NMR to peptide structures at natural abundance. 

            (iv)             Understanding protein folding from synthetic design of specific motifs.

            (v)               3D-structure elucidation of proteins and peptides.

            (vi)             Metabolomics studies on stem cell growth media.


 Following are the highlights of some of these projects:   

 

(i) Amyloid peptide fragment metal interaction: 

 

Charged residues in Amyloid Ab fragments, Ab16 and Ab12 were shown to be involved in Aluminium binding rather than the usual histidine residues.  NMR structures with and without Aluminium was calculated and binding site identified using modeling. Such studies are useful in inhibiting amyloid formation.

(ii)  (a) Conus peptides : Contryphans and its analogs study (Lo-959).